Figure 4.

Deletion of P2ry12 attenuated the development of tactile allodynia. A, PWT was measured in wild-type and P2ry12^−/− mice before and after L5 spinal nerve transection. Each data point represents the mean ± SEM of PWT (in grams) of four animals from each group (*p < 0.05 vs p2ry12^+/+ group; ^#p < 0.05 vs premeasurement by multiple comparison by Tukey's test after a repeated-measure two-way ANOVA). B–E, L5 spinal cord segments from 14 d postoperative mice were subjected to immunohistochemistry using anti-Iba1 antibody. Dotted lines trace the outline of the dorsal horn. Scale bar, 100 μm. F–I, High-magnification images constructed from a series of z-stack images using the deconvolution technique. Scale bar, 10 μm.