Figure 5.
Yif1B colocalization with 5-HT1AR. A, Transfection of 5-HT1A-eGFP-stable LLC-PK1 cells with Yif1B. Yif1B immunofluorescence is shown in red (anti-Yif1B affinity-purified polyclonal antibody; 1:1000; A1), and eGFP autofluorescence is shown in green (A2). B, Primary cultures of rat hippocampal neurons (DIV 7) were cotransfected with a plasmid encoding the 5-HT1A-eGFPR and Yif1B. Superposition of labels shown in 1 and 2 is visible in 3. In 4, the colocalized pixels are shown in white. Arrows show Yif1B and 5-HT1AR colocalization in insets. Scale bars, 10 μm.