Figure 6.

Downregulation of the protein Yif1B by siRNA in primary cultures of rat hippocampal neurons. A1, A2, Yif1B was detected by Western blots (anti-Yif1B affinity-purified polyclonal antibody; 1:1000) of protein extracts, 48 h after transfection of neurons at DIV 7; α-tubulin was detected by Western blots on the same sample (mouse antibody; 1:1000) to normalize the amount of extract. A1, siRNA(Yif1B-1); A2, siRNA(Yif1B-2). All neurons were cotransfected with a control plasmid (encoding eYFP). “Control” corresponds to eYFP alone, “siRNA(Yif1B-1/2)” to eYFP plus siRNA(Yif1B-1/2), and “siRNA(contr-1/2)” to eYFP plus siRNA(contr-1/2). B, Quantification of Yif1B protein expression (normalized with reference to α-tubulin expression) in transfected neurons (n = 5, in 3 independent experiments). Bars represent mean, and error bars represent SEM.