Figure 7.
Altered 5-HT1A receptor distribution in the distal part of dendrites after Yif1B RNA interference. A, Primary cultures of rat hippocampal neurons (7 DIV) were transfected with a plasmid encoding the 5-HT1A-eGFPR (Control, left), cotransfected with the 5-HT1A-eGFPR plus the siRNA(Yif1B-1) (middle), or cotransfected with the 5-HT1A-eGFPR plus the siRNA(contr-1) (right). Immunofluorescence was performed with anti-GFP antibody to enhance the GFP signal (green, top) or anti-α-tubulin antibody (red, middle). Bottom, Overlay. Note the drastic reduction of 5-HT1A-eGFPR fluorescence in the distal part of dendrites in siRNA(Yif1B-1)- but not in siRNA(Contr-1)-transfected neurons. B, 5-HT1A-eGFPR (green) and tubulin (red) fluorescence profiles along the longest dendrites (arrows) of the corresponding neurons. C, Cumulated fluorescence profiles for each group (60 neurons analyzed). siRNA(Yif1B-1) reduces 5-HT1A-eGFPR fluorescence in the distal part of dendrites without affecting their average length, as shown by the tubulin fluorescence distribution. Scale bar, 50 μm.