Figure 7.

Inhibition of PKC activity by bisindolylmaleimide I reduces the PDBu response. A, Time course of the EPSC amplitude potentiation of dentate gyrus autapses by 1 μm PDBu in the absence or presence of 1.2 μm Bis I during 0.2 Hz stimulation (mean ± SEM). Horizontal bars show the onset and the duration of drug application. ●, Reference PDBu response (−Bis I); N = 24; n = 6 (EPSC₀ = 4.35 ± 0.51 nA, mean ± SEM); ◇, PDBu response in the presence of Bis I (+Bis I); N = 31; n = 3 (EPSC₀ = 4.69 ± 0.49 nA, mean ± SEM). B, PDBu-mediated EPSC amplitude potentiation in the absence (−) or presence (+) of Bis I versus EPSC₀ (mean ± SD). Individual data points were binned in classes of 1 nA for EPSC₀ > 0.5 nA, and mean drug effects were calculated for each class. The continuous line is the fit to the values from Figure 3B (left); ▼, expected reference PDBu response calculated from the EPSC₀ amplitudes of the cells used in the Bis I experiment and the curve fit of the reference PDBu “potentiation curve” (continuous line) (Fig. 3B, left); ♦, mean PDBu response measured in the presence of Bis I; ◇, individual data points measured in the presence of Bis I.