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. 2019 Jul 25;10:1718. doi: 10.3389/fimmu.2019.01718

Figure 4.

Figure 4

Suppression of cytokine production by PHA-767491. (A) PHA-767491 suppresses cytokine production in OT-I CTL. APCs expressing single chain H-2Kb with OVA peptide were seeded into 96 well plates 1 day prior to the experiment. CTL were pre-treated with BFA for 30 min before co-culturing with the APCs for 6 h. The percentages of the positive population of each sample are indicated for each plot. (B) PHA-767491 also suppresses cytokine production in strongly polarized OT-I CTL. For the preparation of the OT-I CTL, OT-I splenocytes were pre-treated with either 10 nM OVA peptide and 10 U/ml IL-2 only, 10 nM OVA peptide and 10 U/ml IL-2 with 10 ng/ml IL-12, or 10 nM OVA peptide and 10 U/ml IL-2 with 10 ng/ml IL-15. CTL were pre-treated with BFA and restimulated by co-culturing with APCs expressing single chain H-2Kb with OVA peptide for 6 h. The percentages of each quadrant in each graph are shown. Data shown is representative of at least three independent experiments. Bar charts are represented as mean ± SEM. Statistical significance was determined by unpaired two-sided Student's t-test (***p < 0.001).