Table 1.

Values of modified Pearson's correlation coefficient of FM4-64 pixels versus acidic organelle and fluorescent protein markers

	r̃12	n	p valuea	Compartment labeled
FM1-43	0.92 ± 0.03	8	ND	FM-labeled compartments
Quinacrine	0.79 ± 0.07	12	ND	Acidic vesicles
Lysotracker Green	0.84 ± 0.09	12	ND	Acidic vesicles
BPB	0.58 ± 0.10	9	ND
EGFP-sialin	0.83 ± 0.09	11	ND	Lysosomes/late endosomes (Morin et al., 2004)
CD63/LAMP3-EGFP	0.68 ± 0.19	12		Lysosomes/late endosomes
Ti-VAMP/VAMP7-EGFP	0.74 ± 0.13	9	ND	Lysosomes/late endosomes (Advani et al., 1999; Martinez-Arca et al., 2000)
VAMP8-EGFP	0.55 ± 0.17	24	<0.05	Early and late endosomes (Metzelaar et al., 1991; Martinez-Arca et al., 2000)
VAMP2-EGFP	0.39 ± 0.18	23	<0.001	Small secretory vesicles (Parpura et al., 1995)
VAMP3-EGFP	0.38 ± 0.23	20	<0.001	Early endosomes/intermediate transport vesicles (Chilcote et al., 1995; Teter et al., 1998)
VGlut1-Venusb	0.31 ± 0.17	14	<0.001	Glutamate-containing synaptic-like microvesicles (Wojcik et al., 2004)
VGlut3-Venusb	0.27 ± 0.22	14	<0.001	Glutamate-containing synaptic-like microvesicles (Seal and Edwards, 2006)
Computer-generated image	0.07 ± 0.02	20	ND	Absence of colocalization

r̃₁₂, Noise-reduced modified Pearson's coefficient (see supplemental material, available at www.jneurosci.org); n, number of cells (or trials).

^aSignificance of difference versus r̃₁₂ value of CD63-EGFP.

^bFor additional explanation of these two markers, see supplemental Figure 9 (available at www.jneurosci.org as supplemental material).