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RhoA activity is decreased by blocking neural activity. The amounts of GTP-bound RhoA and total RhoA were detected by the pull-down assay (inset). Relative RhoA activity (GTP-bound RhoA/total RhoA) (mean and SEM) was calculated for control, TTX-treated, and APV/DNQX-treated explants. *p < 0.05; **p < 0.01.
