Figure 5.
Internalization of sst2A-EGFP receptors in preexisting clathrin-coated pits. A, Time series showing a representative example of the clusterization of sst2A-EGFP receptors at the site of an individual preexisting clathrin-coated pit. Scale bar, 0.2 μm. B, C, Time course of EGFP and Ds-Red fluorescence intensity changes measured at three clathrin-coated pits (B) and at three portions of the membrane between clathrin-coated pits (C) before and during octreotide application. Note that, after agonist treatment, sst2A-EGFP fluorescence dramatically increases at coated-pit spots and decreases at membrane sites between coated-pit spots. Error bars indicate SEM.