Figure 7.
Effect of pertussis toxin or suramin treatment on agonist-induced sst2A receptor internalization and nuclear accumulation of the β-arrestin 1. Neurons were cotransfected with plasmids encoding the wild-type receptor (sst2A-WT) and the β-arrestin 1 tagged with EGFP (β-arrestin 1-EGFP), treated with PTX for 20 h or with suramin for 30 min before octreotide treatment and fixed after 10 min. Localization of sst2A receptors was revealed by immunocytochemistry. Note that pertussis toxin or suramin treatments do not prevent sst2A receptor internalization and nuclear accumulation of the β-arrestin 1 after agonist stimulation. Scale bar, 5 μm.