Figure 3.

Blocking NKCC1 inhibits the GABA_A-mediated increase in intracellular [Ca²⁺]_i in lesioned neurons. A, Fluorescence of Fluo-4 in organotypic hippocampal slices. Scale bar, 100 μm. B, In unlesioned slices, application of glutamate but not muscimol evokes an increase in [Ca²⁺]_i. C, In lesioned slices, 100 μm muscimol evokes an increase in [Ca²⁺]_i. This effect can be blocked by NKCC1 inhibitor bumetanide (10 μm) (D). E, In BDNF-treated unlesioned slices, muscimol evokes an increase in [Ca²⁺]_i, which is blocked by bumetanide. F, In cut slices treated with BDNF, muscimol does not evoke an increase in [Ca²⁺]_i. n = 4. Error bars indicate SE.