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Distribution of pCREB-positive neurons after single-cell stimulation. a, CREB phosphorylation produced by neuronal stimulation (40 spikes at 10 Hz) in current clamp is distributed in the culture and can be observed at a distance from the stimulated cell. b, CREB phosphorylation produced by neuronal stimulation (20 DP of 50 mV at 0.1 Hz) in voltage clamp in the presence of TTX is limited to the neurons surrounding the stimulated cell. Squares show nuclei at higher magnification.
