Table 3.

Single-channel properties for wild-type and T686A GluR2 channels

	GluR2-wt		T686A
	Glutamate	Quisqualate	Glutamate	Quisqualate
Conductance (pS)
O1	6.5 ± 0.2	7.5 ± 0.2	6.1 ± 0.2	6.4 ± 0.4
O2	14.8 ± 0.2	15.4 ± 0.3	14.5 ± 0.2	14.7 ± 0.2
O3	23.7 ± 0.4	24.2 ± 0.3	23.5 ± 0.3	24.0 ± 0.5
O4	36.2 ± 0.7	36.8 ± 0.2	36.9 ± 0.7	36.6 ± 0.6
Relative occupancy (%)
O1	46.7 ± 3.3A	35.6 ± 8.9a	71.5 ± 2.2	55.4 ± 6.0a
O2	32.4 ± 2.5a	33.8 ± 3.1a	23.4 ± 2.3	27.3 ± 1.1
O3	19.1 ± 1.5A	27.3 ± 7.1a	4.7 ± 0.8	15.1 ± 4.1a
O4	2.0 ± 0.4A	4.0 ± 2.2	0.4 ± 0.2	2.3 ± 1.2
Shut-time distributions
τ 1 (ms)	0.4 ± 0.1A	0.3 ± 0.1A,B	2.1 ± 0.1	1.2 ± 0.3a
area (%)	(30 ± 2.9)	(28 ± 3.4)	(45.7 ± 5.5)	(32 ± 4.2)
τ 2 (ms)	3.6 ± 0.5A	2.7 ± 0.5a	18.3 ± 4.4	8.1 ± 2.8
area (%)	(22 ± 2.9)	(19 ± 2.9)	(24 ± 1.2)	(27 ± 1.9)
τ 3 (ms)	60.9 ± 11	57 ± 11	155 ± 35	73 ± 13
area (%)	(21 ± 3.3)	(27 ± 5.6)	(25 ± 5.3)	(24 ± 4.5)
τ 4 (ms)	396 ± 85	384 ± 66	729 ± 98	459 ± 155
area (%)	(27 ± 3.5)	(29 ± 4.3)	(5.7 ± 0.9)	(16 ± 2.6)

Single-channel currents were recorded in outside-out patches in response to glutamate (10 mm for GluR2-wt, 50 mm for T686A) or quisqualate (0.5–1 mm for GluR2-wt, 5 mm for T686A). Mean ± SEM values from four to six patches. If F ratio tests indicated the results were homogeneous with respect to variance, comparisons of the mean values in each row were made with one-way ANOVA. When this was not the case, pairwise comparisons were made with two-tailed Student's t test assuming unequal variance. Lowercase and uppercase superscripts indicate significance at p < 0.05 and 0.01, respectively.

^a,^ADifferent from corresponding T686A results with glutamate.

^b,^BDifferent from corresponding T686A results with quisqualate.