Figure 7.

Angpt2 is expressed throughout the brain during endotoxemia, mainly by endothelial cells and rarely by astrocytes. a–d, Dark-field micrographs showing in situ hybridization signals for Angpt2 mRNA in brain sections of mice killed at different times after intraperitoneal LPS injection. Abbreviations: CC, Corpus callosum; Cor, cerebral cortex; CP, caudoputamen; LV, lateral ventricle. Scale bar: a–d, 250 μm. e, Bright-field image showing hybridization signals (black grains) for Angpt2 mRNA at higher magnification. Blue, Thionin counterstaining. Scale bar, 20 μm. f, Increased levels of Angpt2 mRNA were detected by real-time PCR in the brains of mice killed 6 h after LPS injection. Data are expressed as a ratio to 18S rRNA *Significantly different from the other groups (two-way ANOVA: treatment effect, p < 0.0001; time effect, p < 0.0001; interaction, p < 0.0001). Error bars indicate SEM. g–i, Double labelings for Angpt2 mRNA (black grains; in situ hybridization) and different cell-specific markers (red-brown; immunoperoxidase staining). The arrows indicate double-labeled cells. Scale bar: g–i, 20 μm.