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. 2008 Aug 20;28(34):8624–8634. doi: 10.1523/JNEUROSCI.1957-08.2008

Figure 2.

Figure 2.

Effects of an overexpression of TFAM by the tetracycline-off system on rotenone-induced intracellular ROS generation and NF-κB nuclear translocation in HeLa cells. A, CLMS images of rotenone-induced intracellular ROS generation measured using a ROS-sensitive dye (green), DHE, in HeLa cells cultured with or without DC. Scale bar, 20 μm. B, The mean DHE oxidation levels in HeLa cells cultured with or without DC after treatment with EtOH or rotenone. Each column and bar represent the mean ± SEM of six experiments. The asterisks indicate a significant difference between two groups (*p < 0.05; **p < 0.01; ***p < 0.001). C, NF-κB (green) and propidium iodide (PI)-stained nuclei (red) in HeLa cells cultured with or without DC after the treatment with EtOH or rotenone. The arrowheads show HeLa cells with the nuclear translocated NF-κB. Scale bar, 50 μm. D, The mean percentage of the NF-κB nuclear translocation in HeLa cells cultured with or without DC after the treatment with EtOH or rotenone. Each column and bar represent the mean ± SEM of nine experiments. The asterisks indicate significant differences between two groups (*p < 0.05; ***p < 0.001). Rot, Rotenone.