Figure 1.

Expression of NO-GC in the spinal cord. A, B, Immunohistochemistry of the GCβ1 subunit of NO-GC in the lumbar spinal cord in wild-type mice (A) and in GC-KO mice (B) reveals specific NO-GC expression throughout the spinal cord with strong immunoreactivity in the dorsal horn and around the central canal. Scale bar, 100 μm. C, Western blot of GCβ1 (70 kDa) with spinal cord homogenates of wild-type mice (left) and GC-KO mice (right) confirms the specificity of the antibody. GAPDH (36 kDa) was used as loading control.