Figure 5.

NO-, CO-, and cGMP-induced nociceptive behavior in GC-KO mice. A–C, Time course of mechanical allodynia induced by intrathecal administration of NOC-5 (NO donor, 50 nmol; A), CORM-A1 (CO-releasing molecule, 40 nmol; B), or 8-pCPT-cGMP (cGMP analog, 15 nmol; C). No allodynia was observed in GC-KO mice after administration of NOC-5 or CORM-A1, whereas 8-pCPT-cGMP-induced allodynia was intact. The hindpaw withdrawal latency times after mechanical stimulation were normalized to baseline latency times and are presented as mean percentage ± SEM. *p < 0.05, comparing GC-KO and WT mice; n = 6 per group.