Figure 4.

Effects of GTRAP3-18 antisense oligonucleotides on GSH contents of CGNs. A, CGNs were transiently cotransfected with pGFP and GTRAP3-18 sense or antisense oligonucleotides. Two days later, immunocytochemistry using anti-GTRAP3-18 antibody (DsRed) was performed. We considered GFP-positive cells to be oligonucleotide-transfected cells. B, CGNs were transiently cotransfected with pDsRed and GTRAP3-18 sense or antisense oligonucleotides. Two days later, the cells were stained with CMFDA (CMF; green). We considered DsRed-positive cells to be oligonucleotide-transfected cells. C, The fluorescence intensity on the oligonucleotide-transfected cells (GFP-positive or DsRed-positive cells) was quantified in two independent experiments with triplicate culture wells. The average number of GFP- or DsRed-positive cells was 5–10. *p < 0.05 compared with GTRAP3-18 sense oligonucleotide-transfected cells.