Figure 8.

Effects of siRNA on GTRAP3-18 or EAAC1 expression and GSH contents in vivo. Adult male C57BL/6J mice (n = 3) were treated intracerebroventricularly for 1 week with GTRAP3-18 siRNA, EAAC1 siRNA, or nonsilencing siRNA (20 mg/ml; flow rate, 0.5 μl/h). A, Immunoblot analysis of the mouse hippocampus was performed using each specific antibody. B, Levels of protein bands were quantified against the amounts of actin in the same lysates. C, GSH contents of the mouse hippocampus were measured by the NADPH-dependent GSH reductase method. *p < 0.05 compared with vehicle. Error bars indicate SEM.