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Testosterone treatment stimulated MMP2 transcription in vivo. Quantitative RT-PCR of HVC mRNA derived from testosterone-treated birds revealed that MMP2 levels rose by 4 d (T4) after testosterone and had at least doubled by 8 d; in contrast, MMP9 levels were not affected by testosterone treatment. *p < 0.05; **p < 0.01; ***p < 0.001 by ANOVA with Bonferroni t tests.
