Figure 7.
Blocking CaV2.2–laminin β2 interactions in vivo leads to increased innervation of the skin. A–C, Agarose beads soaked in 10 μm CaV2.1 or CaV1.2 11th extracellular loop peptide were implanted in stage 16 embryos. After 1 d, stage 34 embryos were fixed and stained with anti-HNK-1 to reveal sensory processes and sensory nerve terminals innervating the skin as in Figure 6. Scale bar, 50 μm. D, Embryos exposed to CaV2.1 loop peptide (C) exhibited significantly greater numbers of sensory nerve terminals than embryos exposed to CaV1.2 loop peptide (B) (*p < 0.05; n ≥ 3 embryos for each condition). Error bars indicate SEM.