Figure 2.
Generation of human TTBK1 transgenic mice. A, Schematic presentation of the 57 kb TTBK1 genome consisted with 5′-flanking region (containing repetitive sequences and CpG island), 14 exons (numbered boxes) and 13 introns (containing two Alu repeats in introns 1 and 12), and additional CpG island in exon 14. B, Northern blotting of mouse endogenous TTBK1 mRNA in multiple tissues. C, Immunoblotting of transgenic human TTBK1 (TTBK1-Tg, top) and endogenous mouse TTBK1 (non-Tg, bottom) in multiple tissues using anti-TTBK1 mAb.