Expression of TTBK1 and phospho-NF in TTBK1-Tg mouse brain. A–H, TTBK1 staining of sagittal brain sections of aged non-Tg (A–D) or TTBK1-Tg mice (E–H) at 12–13 month-old. A, E, hippocampus; B, F, subiculum; C, G, visual cortex; D, H, external pyramidal layer of visual cortex. I–P, phospho-NF staining of non-Tg (I–L) and TTBK1-Tg mice (M–P) in the same age. I, M, entorhinal cortex; J, N, subiculum; K, O, visual cortex; L, P, the internal and external pyramidal layers of visual cortex. Original magnifications: A, C, E, G, I, K, M, O, 100×; B, D, F, H, J, L, N, P, 200×. Scale bars: (A–D, I–L), 100 μm.