Figure 7.

Caspr, but not neurofascin, distribution is altered in long-term DCC-deficient cultures. Long-term (60 DIV) cerebellar slice cultures were triple-labeled with antibodies against NFH, MBP, and either nfc (A–D) or Caspr (E–H). MBP was visualized using Alexa 488-conjugated secondary antibodies (green), NFH was visualized using Alexa 633-conjugated secondary antibodies (blue), and nfc or Caspr were visualized using Alexa 546-conjugated secondary antibodies (red). The length of nfc-immunoreactive bands were unchanged between DCC^−/− and DCC^+/+ nodal regions, whereas Caspr-immunoreactive domains were lengthened at DCC^−/− paranodes relative to wild-type slices (supplemental Table 5, available at www.jneurosci.org as supplemental material). Magnification: 100× objective; digital zoom, 4. Scale bar, 2 μm.