Figure 3.

The functional blockade of endogenous extracellular S100B reduces KA-induced gamma oscillations in WT mice. A, Schematic diagram of the experiment. Function of endogenous extracellular S100B (orange particle) is blocked by applied anti-S100B antibody (slash) in WT mice. As, Astrocyte; Py, pyramidal cell. B, Representative traces of field potential recording shows that gamma oscillations in CA1 stratum radiatum after KA is smaller in anti-S100B (0.2 mg/ml) infusion side. C, Local infusion of anti-S100B antibody significantly decreases gamma-band amplitude from 10 to 60 min after KA injection in WT mice (mean ± SEM; n = 8). D, Group comparison of the normalized gamma-band amplitudes shows similar amplitudes in 0–10 min before KA injection (left) but decreased amplitudes in anti-S100B infusion side 40–50 min after KA injection in WT mice (right graph; mean ± SEM; **p < 0.01; two-tailed paired t test). E, Local infusion of anti-S100B antibody has no effect on the development of gamma oscillations in S100B(−/−) mice (mean ± SEM; n = 7). F, Group comparison of the normalized gamma-band amplitude for before and after administration of KA in S100B(−/−) mice shows no effect of anti-S100B antibody (mean ± SEM).