Figure 4.

WIN and AM1241 activate the TRPA1 channel expressed in CHO cells. Representative traces show activation of TRPV1 and TRPA1 by ACEA and WIN or AM1241, respectively. CAP and MO were used as control stimuli for TRPV1 and TRPA1, respectively. Agonists were applied sequentially to CHO cells expressing pcDNA3 (marked as CHO cells), GFP, TRPV1, TRPA1, or the combination of TRPV1 and TRPA1. The horizontal bars mark duration of agonist application. Types and concentrations of agonists are denoted above horizontal bars. Recordings were done in whole-cell patch voltage-clamp configuration with SES and SIS as external and internal solutions.