Figure 3.

Map of targeted open reading frames (ORFs) and diagram of PCR strategy. Nested primers (black triangles) were used to amplify parts of the UL55 or UL56 ORFs. The amplified fragments are represented by thin solid lines between the primer binding sites. Fragments were sequenced and assembled to final contiguous sequences of 0.6 and 2.3 kbp. At the top of the figure, the genomic locus spanning ORFs UL55 and UL56 is depicted with open arrows. The arrowhead indicates the direction of transcription. The start of the ruler corresponds with the first base of the ORF UL56.