Figure 2.

Ethanol consumption during pregnancy increases the number of GABA-immunoreactive cells in the neocortex. A, Representative GABA-immunoreactive E14.5 telencephalic sections obtained from control (n = 10) and ethanol exposed in utero (n = 7) wild-type littermates of BAC-Lhx6 litters. Note that immunostaining for GABA is clearly evident in the cells in the marginal, intermediate, and subventricular zones of the neocortex. Scale bar, 100 μm. B, C, Ethanol exposure increased the density of GABA-immunopositive cells throughout the extent of the neocortex as seen in cortical bins (B) and in layer density (C). Asterisks denote a significant difference from control (Student's t test; p < 0.01).