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. 2008 Feb 20;28(8):1865–1870. doi: 10.1523/JNEUROSCI.5417-07.2008

Figure 3.

Figure 3.

The NR1 CT does not bind α-actinin-2. A, B, Coomassie-stained gels showing supernatant (Sn), column flow-through (FT), and metal-affinity purified material (P) of 6xHis-tagged NR1 CT coexpressed with the α-actinin-2 rod domain (indicated by arrowhead to left of A) without (A) or with (B) CaM. The NR1 or NR1/CT complex is indicated by a bracket to right of B. C, Same as in A, but with α-actinin-2 rod domain plus α-actinin-2 CT. D, Same as in C, but with 6xHis-tagged NR2B CT (amino acids 1120–1482). Arrow denotes successful copurification of the α-actinin-2 rod plus CT domains with NR2B. On right is shown a metal-affinity purification of 6xHis-tagged α-actinin-2 rod plus CT domains.