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Knock-down of endogenous angiogenin potentiates stress-induced cell death in motoneurons in vitro. A, Western blot analysis demonstrating efficient knock-down of murine ANG1 in NSC34 cells with siRNAs 48 h after transfection. B, Apoptotic cell death of primary motoneuron cultures transfected with murine ANG1 siRNA and treated with AMPA was assessed after Hoechst 33258 staining. (*p < 0.01 vs nontransfected sister cultures, mean ± SEM, n = 7–10).
