Table 1.
PCR primer sequences
| Gene name | Primer | Sequence (5′–3′) |
|---|---|---|
| Sense | 5′-CCCGGGTCAGCTATTGAAGGTCTG-3′ (−473/−449) | |
| pGL2-NR1-473 | Antisense | 5′-GAGCTCCGGGCACAGCGGGCCTGG-3′ (−24/1) |
| Sense | 5′-CATTCACGCCAACGCAGGCGCGCG-3′ (−356/−332) | |
| pGL2-NR1-356 | Antisense | 5′-GAGCTCCGGGCACAGCGGGCCTGG-3′ (−24/1) |
| Sense | 5′-TGCTAACGCGAAAACACACACCCT-3′ (−415/−392) | |
| pGL2-NR1-mHRE1 | Antisense | 5′-AGGGTGTGTGTTTTCGCGTTAGCA-3′ (−415/−392) |
| Sense | 5′-CACACACCCTAAAAGGCGCTCCTC-3′ (−401/−378) | |
| pGL2-NR1-mHRE2 | Antisense | 5′-GAGGAGCGCCTTTTAGGGTGTGTG-3′ (−401/−378) |
| Sense | 5′-TGCTAACGCGAAAACACACACCCTAAAAGGCGCTCCTC-3′ (−415/−378) | |
| pGL2-NR1-mHRE1/2 | Antisense | 5′-GAGGAGCGCCTTTTAGGGTGTGTGTTTTCGCGTTAGCA-3′ (−415/−378) |
| Sense | 5′-ATGAGCACCATGCACCTGCTGACA-3′ (266/290) | |
| pcDNA3.1-NR1-myc/His | Antisense | 5′-GCTCTCCCTATGACGGGAACACAG-3′ (3056/3079) |
| Sense | 5′-TCAAGTCAGCAACGTGGAAG-3′ (577/596) | |
| HIF-1α | Antisense | 5′-TATCGAGGCTGTGTCGACTG-3′ (755/774) |
| Sense | 5′-TGACAACTTTGGCATCGTGGAAGG-3′ (565/588) | |
| GAPDH | Antisense | 5′-CAACGGATACATTG GGGGTAGGAAC-3′ (774/797) |
| Sense | 5′-TCCACCAAGAGCCCTTCGTG-3′ (1473/1492) | |
| NR1 | Antisense | 5′-GGCCCCGTACAGATCACCTT-3′ (1562/1681) |