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Synaptic frequency characteristics depend on the EPSC amplitude. A, B, Relationship between the mean EPSC amplitude and the paired pulse ratio in L2/3 to L5 connections (n = 31) in A and L5 to L5 connections (n = 49) in B. Ten to twenty recordings of EPSCs evoked by two successive presynaptic spikes (10 Hz) were averaged in individual connections. The paired pulse ratio was calculated by the mean of the second response divided by the mean of the first EPSC amplitude.
