Figure 4.

Interaction of Rab7 wt and mutant proteins with RILP. L40 yeast cells were cotransformed with pGADGH-RILPC33 and the indicated pLexA-Rab7 wt or mutant constructs. The β-galactosidase activity of double transformants was measured using o-nitrophenyl-β-d-galactoside as substrate as detailed in Materials and Methods. Activities are measured as arbitrary relative units and represent mean ± SEM values of six independent transformants. Differences between the values of Rab7 CMT2B mutated proteins and that of Rab7 wt are statistically significant at p values <0.05 (*) or <0.01 (**).