Figure 6.

vMIP-2, a broad-spectrum chemokine receptor antagonist, prevents neuronal cell death in the CA1 region after TFI. A, Bright-field photomicrographs of the CA1 section stained with cresyl violet after sham operation (Ctrl), at 3 d after forebrain ischemia alone (FI), or in the presence of trolox or vMIP-2. Scale bar, 200 μm. B, Viable neurons at 3 d after FI with an intracerebroventricular injection of 1 μg of vMIP-2 at 0, 12, and 24 h after reperfusion in the CA1 region and at 7 d after FI with vMIP-2 treatment were analyzed and presented as means ± SEM (n = 4 animals for each condition). *Significantly different from the relevant control at p < 0.05 using ANOVA and Student–Newman–Keuls analyses. C, Fluorescent photomicrographs of CA1 sections labeled with a MAP-2 antibody after sham operation (Ctrl), 3 d after forebrain ischemia alone (FI), or in the presence of trolox (50 mg/kg, i.p.) immediately after reperfusion. Scale bar, 250 μm.