Figure 4.
Increased KCC2-ir underlies the negative shift in EGABA in P10 female CON and male KA456 and SS456 groups. A, Male and female rats; representative photos (400× magnification) of CA1 pyramidal neurons from P10 CON rats using a KCC2 specific antibody, counterstained with Novared substrate (brown–red color). Increased KCC2-ir is present in female CA1 pyramidal neurons. B, Male P10 CON, KA456, and SS456 CA1 pyramidal neurons stained with KCC2-specific immunochemistry, using Novared substrate. High levels of KCC2-ir is present in KA456 rats, intermediate in SS456, and low in CON. C, Male and female rats; the results of densitometric analysis of KCC2-ir in CA1 neurons of the three treatment groups are depicted as means ± SEM. Significant sex differences are documented among CON groups. In males only, both KA456 and SS456 have increased KCC2-ir compared with CON (KA456 > SS456 > CON). PN, Postnatal day. Scale bar, 100 μm.