Figure 2.

Microtubules invade spines consistently over 1 h of imaging. a, Representative graphs of MT entry into spines as a function of time. The x-axis is time (0–60 min) and the y-axis is the ratio of invasion (0.0–1.0, except during tSHPs in which values vary from 1.10 to 1.22 for examples shown here). The asterisks above the invasions in the first graph indicate tSHPs. b, Cumulative plot of all MT invasions from 69 spines (binned in 10 min intervals). Error bars are ± SEM. There was neither a difference between any of the data points over the 1 h imaging interval (p = 0.567, repeated measures ANOVA) nor a linear trend over time (p = 0.189, post test for linear trend).