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Refined transmembrane topology of VGAT. A, Transmembrane structure of VGAT as proposed by McIntire et al. (1997) and as predicted by the TMHMM algorithm (B). Transmembrane domains are marked in gray. Epitopes in green were used to produce antibodies. Epitopes in red were detected by mass spectrometry. Insets denote the predicted exposure of luminal VGAT epitopes at the plasmalemmal surface and the likelihood of their targeting by antibodies during SV recycling.
