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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Mol Cell. 2019 Apr 17;74(6):1148–1163.e7. doi: 10.1016/j.molcel.2019.03.025

Figure 2. Validation confirms TAF5L and TAF6L are the new epigenetic genes for the mESC state.

Figure 2

(A) RT-qPCR data showing mRNA expression levels of Tada1, Tada3, Taf5l and Taf6l in their corresponding KOs compared to wild-type. mRNA levels were normalized to GAPDH. Paired sgRNAs were used to target exon/s at the N-terminal of Tada1, Tada3, Taf5l and Taf6l candidate epigenetic genes to create homozygous/biallelic deletion or KO clones.

(B) Endogenous Oct4 mRNA expression levels in the Tada1, Tada3, Taf5l and Taf6l KOs.

(C) Immunofluorescence staining of OCT4 in the Tada1, Tada3, Taf5l and Taf6l KOs. Scale bar is 100µm.

(D) Quantification of mean fluorescence intensity (MFI) of OCT4 in the Tada1, Tada3, Taf5l and Taf6l KOs.

Data are represented as mean ± SEM (n = 3); p-values were calculated using ANOVA. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; and ns (non-significant).

Related to Figure S2.