Table 1.
Primer sequences used for RT-PCR analyses of NADPH oxidases in microglia
| Primer | Sequence (5′ to 3′) | Amplicon (bp) | Accession number |
|---|---|---|---|
| Nox2_for | CGAAGACAACTGGAAGGAA | 956 | NM_007807 |
| Nox2_rev | GCTCCCACTAACATCACCAC | ||
| Nox3_for | TTGTGGCACACTTGTTCAACCTGG | 493 | AY573240 |
| Nox3_rev | TCACACGCATACAAGACCACAGGA | ||
| Nox4_for | ATCACAGAAGGTCCCTAGCA | 615 | NM_015760 |
| Nox4_rev | GGTCCAGAAATCCAAATCCA | ||
| Duox1_for | CACCATTGGGACCCTTTGCTGTTT | 761 | XM_130483 |
| Duox1_rev | AGCCTTTCATGAAGACCACCAGAA | ||
| Duox2_for | AACCACCTATGTGGGCATCATCCT | 491 | XM_619808 |
| Duox2_rev | AGCTGCCATGGATGATGATCAGGA | ||
| Rac1_for | TGTGGTGGTGGGAGACGGAG | 528 | NM_009007.1 |
| Rac1_rev | TTCTCTGTCCCCCTCCTGTC | ||
| Rac2_for | GACAGGAGGGGGACAGAGAA | 254 | NM_009008.3 |
| Rac2_rev | AGCGAGAAGCAGATGAGAAA | ||
| p22phox_for | GCTGCCCTCCACTTCCTGTT | 254 | NM_007806 |
| p22phox_rev | GCTGCCTCCTCTTCACCCTC | ||
| p40phox_for | AGAGCGACTTTGAGCAGCTT | 460 | AB002665 |
| p40phox_rev | TGTGGAGACACACCCTTGAT | ||
| p47phox_for | AGCCTGAGACATACCTGGTG | 446 | AB002663 |
| p47phox_rev | AGACTTCTGCAGATACATGG | ||
| p67phox_for | CAAGGCTACGGTTGTAGCAT | 465 | AB002664 |
| p67phox_rev | ACCTTGAGCATGTAAGGCAT |
Accession numbers of the sequences and size of the corresponding amplicons are indicated. For, Forward; rev, reverse.