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Tonic inhibition is also observed in KCl-based intracellular solution and is enhanced by low concentrations of GABA. A, At resting membrane potential and in the presence of extracellular TTX (1 μm), this SPN was hyperpolarized by 100 μm bicuculline. B1, Recording from another SPN where 100 μm bicuculline elicited a small hyperpolarization. B2, In the presence of applied GABA, bicuculline caused a larger shift in membrane potential.
