Figure 4.
Fewer precursor cells are present in the dentate neuroepithelium and the migratory stream at E16.5. Expression of Ngn2, Lef1 and Math3 that mark granule precursors was analyzed in control and AtrxFoxg1Cre hippocampus at E16.5 by in situ hybridization using specific DIG-labeled antisense probes. Fewer precursor cells are localized at the dentate notch (indicated by asterisks) and fewer Ngn2+ and Lef1+ precursors are present in the migratory stream at E16.5 (indicated by arrows). Scale bar, 200 μm.