Skip to main content
. 2008 Nov 26;28(48):12759–12764. doi: 10.1523/JNEUROSCI.2439-08.2008

Figure 2.

Figure 2.

Perturbation of rRNA transcription activity in TIF-IACaMKCreERT2 mutants. A, Diagram showing the procedure to induce Cre recombinase activity by tamoxifen intraperitoneal injection and the time course of the analysis. B, Diagram showing the localization of the probes for the 47S leader and the 28S rRNA sequence on the rDNA and the final rRNA products. C–F, Nonradioactive in situ hybridization on coronal paraffin sections from control (C, E) and TIF-IACaMKCreERT2 mutant mice (D, F) probed for the 47S pre-rRNA (C, D) and 28S rRNA (E, F) 4 weeks after tamoxifen injection. Insets show high magnification within the boxed field. G, H, Nonradioactive in situ hybridization on coronal paraffin sections from control (G) and TIF-IACaMKCreERT2 mutant mice (H) probed for the 28S rRNA 3 months after tamoxifen injection. I, J, Immunohistochemistry with NPM/B23 specific antibody is used to analyze the nucleoli in control (I) and in the TIF-IANesCre mutant (J) 3 months after tamoxifen injection. K–P, Double immunofluorescence analysis showing NPM/B23 (red) and p53 (green) in TIF-IACaMKCreERT2 and control 3 months after tamoxifen. Scale bars: (C–F) 500 μm; (G–J) 125 μm; (K–P) 50 μm; (insets) 40 μm.