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. 2008 Feb 27;28(9):2261–2273. doi: 10.1523/JNEUROSCI.4372-07.2008

Figure 5.

Figure 5.

Proliferation of support cell subpopulations is differentially affected by Notch inhibition. A, BrdU pulse-fix time course graph of SC proliferation in control and regenerating neuromasts with 50 μm DAPT or 0.5% DMSO vehicle incubation. B, BrdU pulse-fix time course graph after 400 μm neomycin treatment with 50 μm DAPT (triangle) or 0.5% DMSO (circle) vehicle incubation. BrdU-labeled SCs were subdivided as internal (solid lines, solid shape) or peripheral (dotted lines, open shape) based on neuromast cell position. DAPT incubation increased the number and duration of internal SC proliferation, whereas peripheral cells decreased in proliferation. C, BrdU pulse-fix time course graph of mock-treated control siblings with 50 μm DAPT (triangle) or 0.5% DMSO (circle) vehicle incubation. Both internal (solid lines, solid shape) and peripheral (dotted lines, open shape) SC proliferation decreased with DAPT incubation compared with the vehicle control, but effects were difficult to interpret because of the small numbers of BrdU-positive cells observed. Error bars represent ±1 SEM (n = 10 fish per condition, 7 neuromasts per fish for all 3 graphs) (A–C).