Figure 3.

Fluorescence microscopy for mitochondrial localization in targeted GFP transgenic mice. Aa, Ad, Mitochondrial distribution in the CA1 and CA2 regions of hippocampus in unexposed mice. Ab, Ae, CA1 and CA2 regions of hippocampus with increased mitochondrial fluorescence 24 h after hypoxia. Ac, Af, The CA1 and CA2 regions of hippocampus showing increased mitochondrial fluorescence 48 h after hypoxia. Ba and Bb show scattered fluorescence in the cortex that is similar before (a) and 24 h after (b) hypoxia, whereas the hippocampus in the same mice (c) shows a generalized increase after hypoxia (d). Original magnification, 400×.