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PKA phosphorylation in brain subcortex after hypoxia. Western analysis for total and phosphorylated PKA catalytic subunit in brain nuclear extracts of Wt (A), eNOS−/− (B), and nNOS−/− (C) mice. For all three strains, brain nuclear extracts of controls (0 h) and mice after 6 h hypoxia were probed with phospho-PKA antibodies and compared with total PKA and tubulin expression.
