Figure 1.

In situ hybridization of iPLA₂β mRNA in the nervous system. A–H, The pattern of iPLA₂β mRNA expression in the cerebral cortex (A, B), the cerebellum (C, D), the spinal cord (E, F), and the dorsal root ganglion (DRG) (G, H) of 7-week-old female mice. The antisense probe detected signals in all cortical layers, except for layer I, of the cingulate (cg), the motor (mo) cortex, and the somatosensory (ss) cortex, in the Purkinje cell layer (pl) and the granular layer (gl), but not the molecular layer (ml) of the cerebellum, in the dorsal (do) and ventral (ve) horns of the spinal cord, and the soma-rich region of the DRG (so). No signals were detected in the corpus callosum (cc) or in the axon-rich region (ax) that contains mainly axons and oligodendrocytes (cc) or Schwann cells (ax). The sense probe was used as a negative control (B, D, F, H). Scale bars: A–F, 500 μm; G, H, 180 μm.