Figure 10.

Salubrinal protects select motoneuron signaling proteins. Confocal photomicrograph of facial motoneurons. Preservation of cholinergic dendritic (Alexa Fluor 594; red color) projections with salubrinal (A, A′, A″) is readily evident in comparison with vehicle-treated mice (B, B′, B″). Moreover, salubrinal increased MAP-2 (Alexa Fluor 488; green color) under conditions of LTIH in both VII (vehicle, 1.4 ± 0.04, vs salubrinal, 1.6 ± 0.05; t = 2.8; p < 0.05) and XII (vehicle, 1.3 ± 0.02, vs salubrinal, 1.6 ± 0.05; t = 4.3; p < 0.001). An arrow highlights a dendrite with colocalization of ChAT (A′′, B′′) and MAP-2 (A′, B′). The supplemental material (available at www.jneurosci.org) provides a three-dimensional reconstruction movie of the z-stacks from which the images were obtained. Scale bar, 30 mm.