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. 2008 Dec 17;28(51):13742–13753. doi: 10.1523/JNEUROSCI.4844-08.2008

Figure 6.

Figure 6.

Function of gcm on postembryonic development of perineurial and neuropil glia. A, B, Cells labeled with GFP driven by gcm-GAL4 in a wandering larva. C, D, Cells labeled by MARCM system with gcm-GAL4. Mitotic recombination was induced in NHL. Glial nuclei were labeled with anti-Repo antibody (magenta, A–D). High-magnification images of A and C are shown in B and D, respectively. Note that gcm-positive cells were labeled with anti-Repo antibody (B, D). E–H, Wild-type (E, G) and gcm ΔP1 (F, H) MARCM clones of neuropil glia (E, F) induced at NHL and perineurial glia (G, H) induced at mid-first-instar larvae, respectively. I, J, Quantification of neuropil glia (I) and perineurial glia (J) that were labeled by MARCM system. Average number of neuropil glia per brain (I) and average number of large perineurial glia cluster (>10) per brain were examined in wt, gcm ΔP1, and Df(2L)132 clones. Scale bars, 50 μm. Genotypes: gcm-GAL4/UAS-GFP (A, B); hs-FLP/+; gcm-GAL4, FRTG13, UAS-nlsGFP/FRTG13, tubP-GAL80, repo-GAL80 (C, D); hs-FLP, UAS-mCD8::GFP/repo-GAL4; FRT40A, tubP-GAL80/FRT40A or FRT40A, gcm ΔP1 (E–H); and hs-FLP/repo-GAL4; FRT40A, tubP-GAL80/FRT40A or FRT40A, gcm ΔP1 or FRT40A, Df(2L)132; UAS-nlsGFP/+ (I, J).