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. 2019 Aug 1;9:11210. doi: 10.1038/s41598-019-47769-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Surface and intracellular staining of Dectin-1 ligands. Recombinant Mala s13 and hTrx were detected inside moDC 10–20 min after incubation by a fluorescently labelled anti-6xHistidine-tag antibody. Prior to that, surface staining applying the same antibody was performed to block surface-bound antigens. Exemplary stainings are shown as indicated. w/o: medium control. Plotted graph shows arithmetic mean and SEM of six independent experiments, medium control is set to 1.