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. 2019 Aug 1;10:3460. doi: 10.1038/s41467-019-11494-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Identification and analysis of bacteriocin biosynthesis regions. a Agar overlay assays analyzing growth inhibition activity of BoCL02T12C04 and four transposon mutants against B. thetaiotaomicron VPI-5482 and B. vulgatus ATCC 8482. b ORF maps of a four-gene genetic region common to BoCL02 and BtCL15 showing the insertion sites of the transposon mutants (vertical blue arrows) that abrogated toxin production. Gene names of the BtCL15 strain beginning with the prefix EH213_0 are shown inside each gene. A similar genetic region from the BvCL01 and BvCL14 strains is shown below and the percent similarity of the proteins of these two regions is shown. Putative functions or properties of the proteins encoded by each gene are indicated. c Agar overlay assays analyzing the effects of deletion of three genes in the toxin biosynthesis region on the ability of BtCL15 to inhibit the growth of the two sensitive strains. d Agar overlay assays testing for the ability of the genes encoding the five transmembrane protein (p1845) or the thiol oxidoreductase (p1843) to protect B. thetaiotaomicron VPI-5482 when expressed in trans. e Agar overlay assay analyzing the ability of wild-type BtCL15 and the putative peptide bacteriocin gene deletion (Δ1844) to inhibit the growth of Δ1844. f Agar overlay assay analyzing the production of toxin from E. coli expressing the four-gene region (EH213_1845-1842) from an IPTG inducible promoter